3D Bioplotter Research Papers

3D printing of cell laden bioinks has the potential to recapitulate the hierarchical and spatial complexity of native tissues. However, the addition of cells can alter physical properties of printable resins, which in turn may impede or induce cellular sedimentation or affect the printability and shape fidelity of the final construct. In this study we investigated these considerations by bioprinting gelatin methacrylate (GelMA) bioinks, loaded with various concentrations of mouse fibroblast cells (L929), using extrusion-based direct-write 3D printing (EDP). The impact of various cellular concentrations on viscosity, and temperature-driven gelation of GelMA was examined with a rheometer. The effect of…

While articular cartilage defects affect millions of people worldwide from adolescents to adults, the repair of articular cartilage defects still remains challenging due to the limited endogenous regeneration of the tissue and poor integration with implants. In this study, we developed a 3D-printed scaffold functionalized with aggrecan that supports the cellular fraction of bone marrow released from microfracture, a widely used clinical procedure, and demonstrated tremendous improvement of regenerated cartilage tissue quality and joint function in a lapine model. Optical coherence tomography (OCT) revealed doubled thickness of the regenerated cartilage tissue in the group treated with our aggrecan functionalized scaffold…