3D Bioplotter Research Papers
Fabrication of a bio-instructive scaffold conferred with a favorable microenvironment allowing for superior implant osseointegration and accelerated in situ vascularized bone regeneration via type H vessel formation
The potential translation of bio-inert polymer scaffolds as bone substitutes is limited by the lack of neovascularization upon implantation and subsequently diminished ingrowth of host bone, most likely resulted from the inability to replicate appropriate endogenous crosstalk between cells. Human umbilical vein endothelial cell-derived decellularized extracellular matrix (HdECM), which contains a collection of angiocrine biomolecules, has recently been demonstrated to mediate endothelial cells(ECs) – osteoprogenitors(OPs) crosstalk. We employed the HdECM to create a PCL (polycaprolactone)/fibrin/HdECM (PFE) hybrid scaffold. We hypothesized PFE scaffold could reconstitute a bio-instructive microenvironment that reintroduces the crosstalk, resulting in vascularized bone regeneration. Following implantation in a…
Development of a Photocrosslinkable Methacrylated Methylcellulose and Gelatin bioink for Cartilage Tissue Regeneration
Articular cartilage disease can cause pain, mobility issues, and disability. Clinical treatment includes microfracture, subchondral drilling, graft transplantation, and eventually total joint replacement implant. However, these approaches can present specific problems and limitations. Three-dimensional (3D) bioprinted scaffolds utilising hydrogels can provide a suitable 3D biochemical and biophysical environment, thus is a promising strategy for cartilage tissue therapy and regeneration. This study aims to develop a new hydrogel bioink with improved printability, mechanical, and biological properties for cartilage regeneration. A photocrosslinkable methacrylated methylcellulose (MCMA) and gelatin (GelMA) hybrid bioink is evaluated in this preliminary investigation. The results showed that methylcellulose and…