3D Bioplotter Research Papers

3D Ti6Al4V-beta-tricalcium phosphate (TCP) hybrid scaffolds with interconnected porous network and controllable porosity and pore size were successfully produced by three-dimensional fiber deposition (3DF). The macrostructure of scaffolds was determined by the 3D design, whereas the micro- and submicron structure were derived from the Ti6Al4V powder sintering and the crystalline TCP powder, respectively. Ti6Al4V-TCP slurry was developed for 3DF by optimizing the TCP powder size, Ti6Al4V-to-TCP powder ratio and Ti6Al4V-TCP powder content. Moreover, the air pressure and fiber deposition rate were optimized. A maximum achievable ceramic content in the Ti6Al4V-TCP slurry that enables 3DF manufacturing was 10 wt%. The chemical…

Articular cartilage disease can cause pain, mobility issues, and disability. Clinical treatment includes microfracture, subchondral drilling, graft transplantation, and eventually total joint replacement implant. However, these approaches can present specific problems and limitations. Three-dimensional (3D) bioprinted scaffolds utilising hydrogels can provide a suitable 3D biochemical and biophysical environment, thus is a promising strategy for cartilage tissue therapy and regeneration. This study aims to develop a new hydrogel bioink with improved printability, mechanical, and biological properties for cartilage regeneration. A photocrosslinkable methacrylated methylcellulose (MCMA) and gelatin (GelMA) hybrid bioink is evaluated in this preliminary investigation. The results showed that methylcellulose and…

Extrusion-based bioprinting of hydrogel scaffolds is challenging due to printing-related issues, such as the lack of capability to precisely print or deposit hydrogels onto three-dimensional (3D) scaffolds as designed. Printability is an index to measure the difference between the designed and fabricated scaffold in the printing process, which, however, is still under-explored. While studies have been reported on printing hydrogel scaffolds from one or more hydrogels, there is limited knowledge on the printability of hydrogels and their printing processes. This paper presented our study on the printability of 3D printed hydrogel scaffolds, with a focus on identifying the influence of…

The aim of this study was to synthesize and characterize self-crosslinked bioactive glass/alginate composite scaffolds, as a kind of potential biomaterial for bone regeneration. The scaffolds were fabricated through a self-crosslinking process of alginate by bioactive glass microspheres provided Ca2+ completely, without any organic solvent, crosslinking agent or binder. The microstructure, mechanical properties, apatite-forming ability, ionic release, adhesion, proliferation and ALP activity of human bone marrow-derived mesenchymal stem cells (hBMSCs) of the scaffolds were evaluated. The results showed that uniform films could be obtained on the surface as well as abundant of crosslinking bridges in the interior of scaffolds. The…

Herein we report for the first time the synthesis of three-dimensional scaffolds in the binary system SiO2–P2O5 exhibiting different scales of porosity: (i) highly ordered mesopores with diameters of ca. 4 nm; (ii) macropores with diameters in the 30–80 μm range with interconnections of ca. 2–4 and 8–9 μm; and (iii) ultra-large macropores of ca. 400 μm. The hierarchical porosity of the resulting scaffolds makes them suitable for bone tissue engineering applications. The chemical nature and mesoporosity of these matrices would allow these scaffolds to act as local controlled delivery systems of biologically active molecules, such as certain drugs to…

3D porous Ti6Al4V scaffolds were directly fabricated by a rapid prototyping technology, 3D fiber deposition (3DF). In this study, scaffolds with different structures were fabricated by changing fiber spacing and fiber orientation. The influence of different architectures on mechanical properties and permeability of the scaffold were investigated. Mechanical analysis revealed that compressive strength and E-modulus increase with decreasing the porosity. Permeability measurements showed that not only the total porosity but also the porous structure can influence the permeability. 3DF was found to provide good control and reproducibility of the desired degree of porosity and the 3D structure. Results of this…

Organ or tissue printing, a novel approach in tissue engineering, creates layered, cell-laden hydrogel scaffolds with a defined three-dimensional (3D) structure and organized cell placement. In applying the concept of tissue printing for the development of vascularized bone grafts, the primary focus lies on combining endothelial progenitors and bone marrow stromal cells (BMSCs). Here we characterize the applicability of 3D fiber deposition with a plotting device, Bioplotter, for the fabrication of spatially organized, cell-laden hydrogel constructs. The viability of printed BMSCs was studied in time, in several hydrogels, and extruded from different needle diameters. Our findings indicate that cells survive…